Coding

Part:BBa_K858004:Design

Designed by: Max Jacobs, Jake Sheppard, Sean Kalra   Group: iGEM12_CU-Boulder   (2012-09-24)


NucB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 32
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 32
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 32
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 32
    Illegal NgoMIV site found at 340
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was biobricked without need for site directed mutations.


Source

This part comes from the Bacillus subtilis strain 168. It is a 411 bp region from base 2652387 to 2652797.

References